Other common uses of flow cytometry include gene expression and Applications Flow cytometry has proved to be an effective method of quantifying this. Contemporary flow cytometers are much smaller, less expensive, more user-friendly, and well suited for high-volume operation. Flow cytometry is used for immunophenotyping of a variety of specimens, including whole blood, bone marrow, serous cavity fluids, cerebrospinal fluid, urine, and solid tissues. Multiparametric phenotypic analysis by flow cytometry allows researchers to study the dynamics of immune signaling in intact cells, and offers many unique.
|Published:||14 April 2015|
|PDF File Size:||17.8 Mb|
|ePub File Size:||14.31 Mb|
Autoimmune neutropenias may develop in patients with autoimmune disorders such as Felty syndrome, systemic lupus erythematosus, and Hashimoto thyroiditis.
Applications of Flow Cytometry
When immune-related, flow cytometry can detect anti-neutrophil antibodies and confirm flow cytometry applications origin of neutropenia, possibly flow cytometry applications the need for a bone marrow procedure. Conversely, the absence of anti-neutrophil antibodies narrows the differential diagnosis to nonimmune causes such as bone marrow failure, myelodysplasia, or marrow-infiltrative processes.
Functional deficiencies of leukocytes can be assessed by flow cytometry. Assays for oxidative burst, phagocytosis, opsonization, adhesion, and structure are available One clinical example measures neutrophil adhesion molecules central to a diagnosis of leukocyte adhesion deficiency syndrome type I This syndrome is characterized by an immunodeficiency related to defective neutrophil and monocyte migration to sites of inflammation.
This receptor complex binds endothelial cell ligands such as intercellular adhesion molecule-1 CD54 flow cytometry applicationswhich is necessary for flow cytometry applications adherence and transendothelial migration 78 Platelet-associated immunoglobulin assays by flow cytometry can be direct or indirect assays, similar to other platelet-associated immunoglobulin immunoassays.
In autoimmune thrombocytopenic purpura, free serum antibodies are not found as frequently as platelet-bound antibodies 80 81 82 In contrast, in cases of alloantibody formation, serum antibodies may be detected without evidence of platelet-associated antibodies Flow cytometry is an excellent method for direct analysis of platelet-bound antibodies, and it has also been shown to be of benefit in detection of free plasma antibodies 81 The use of thiazole orange, a fluorescent dye that binds RNA, allows immature platelets also flow cytometry applications to as reticulated platelets to be quantified 86 87 The reticulated platelet count can be used to determine the rate of thrombopoiesis.
Applications of Flow Cytometry
This measurement can separate unexplained thrombocytopenias into those with increased destruction and those with defects in flow cytometry applications production.
Flow cytometry is a rapid and useful method of obtaining a diagnosis.
Until recently, functional analysis of platelet activation was used primarily in research. Immunophenotyping The most common application performed on the cytometer is immunophenotyping.
Flow Cytometry: Principles and Clinical Applications in Hematology
This technique identifies and quantifies populations of cells in a heterogeneous sample - usually blood, bone marrow or lymph.
These cell subsets are measured by labeling population-specific proteins with a flow cytometry applications tag on the cell surface.
In clinical labs, flow cytometry applications is useful in diagnosing hematological malignancies such as lymphomas and leukemia. Cell Sorting The cell sorter is a specialized flow cytometer with the ability to physically isolate cells of interest into separate collection tubes.
Flow cytometry: principles and clinical applications in hematology.
The cytometer interrogates and characterizes each cell as it passes through the laser. The sorter then uses sophisticated electronics flow cytometry applications fluidics to identify and "kick" the cells of interest out of the fluidic stream into a test tube.
Cell Cycle Analysis Flow cytometry can analyze flow cytometry applications states using fluorescent dyes to measure the four distinct phases of the cell cycle.
Along with determining cell cycle replication states, the assay can measure cell aneuploidy associated with chromosomal abnormalities. Apoptosis Apoptosis, or programmed cell death, is a normal part of the life cycle of eukaryotic cells. Cells die for a variety of reasons: