Endophytic actinomycetes were isolated from 6 different plants collected from Uttarakhand, India. Actinomycetes isolates were screened for their herbicidal activity against Parthenium hysterophorus, Ageratum conyzoides and Bidens biternata. These isolates belong to the seven different genera. The isolation of endophytic actinomycetes from surface-sterilized tissues of 36 plant species was made using humic acid–vitamin (HV) agar as a selection. The culturable diversity of endophytic actinomycetes associated with tropical, native plants is essentially unexplored. In this study, endophytic actinomycetes.


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February 07, endophytic actinomycetes Accepted Date: February 24, ; Published Date: February 27, Citation: J Plant Pathol Microbiol 8: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in endophytic actinomycetes medium, provided the original author and source are credited.

Endophytic Actinomycetes and Their Interactions with Host Plants

The present results correlate the presence of endophytic actinomycetes in medicinal plants by isolating them from three medicinal plants i. Ocimum basillicum, Withania somnifera and Rauvolfia tetraphylla. In the present work surface sterilization method and media was standardized and 32 endophytic actinomycetes were isolated from three medicinal plants.

We assessed the efficiency of four different surface sterilization methods and four endophytic actinomycetes for isolation of endophytic actinomycetes. The method with calcium hypochlorite, sodium hydrogen carbonate and sodium azide New method used was more effective in endophytic actinomycetes epiphytic microorganisms.

Among four tested media starch casein agar SCA found to be best media for the isolation of endophytic actinomycetes. We succeeded in obtaining fast endophytic actinomycetes luxurious growth of pure culture of endophytic actinomycetes on modified ISP-4 M inorganic salt solution agar in comparison with standard ISP-4 S.

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Preliminary antibacterial assay of all isolates were tested by confrontation test. The secondary screening of selected isolates were tested by endophytic actinomycetes diffusion test by using ethyl acetate extract which showed broad spectrum antibacterial activity against test human pathogens.

Based on the morphological and phenotypic characters 12 isolates were identified as Streptomyces endophytic actinomycetes.


This is the first report of successful isolation of endophytic actinomycetes from the said medicinal plants, for using newly formulated surface sterilization method and new comparative study on ISP-4 medium.

Keywords Endophytic actinomycetes; Surface sterilization; Isolation media; 16SrRNA; ISP-4; Endophytic actinomycetes activity Introduction The inclusive and widely accepted definition of endophyte endophytic actinomycetes was given by Bacon and White [ 1 ] is microbes that colonize living internal tissues of plants without causing any immediate, overt negative effects.

Plants constitute vast and diverse niches for endophytic organisms, and closer biological associations may have developed between these organisms and their respective hosts than for epiphytes of soil related organisms [ 2 ] Plant associated bacteria, both endophytic and rhizospheric, are known to have beneficial effects on the host plant [ 3 ].


Endophytic actinomycetes unlike the conventional microbes in culture do not show growth immediately. Typically, a specific media composition and constituents are endophytic actinomycetes for the isolation of distinct endophytes [ 19 ].

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Previously unstudied groups of soil bacteria were isolated endophytic actinomycetes using different types of media [ 19 - 21 ]. The research on endophytic actinomycetes is relatively less because surface sterilization of the samples a very important step, if the sterilization time is too long, the plant cell would be damaged and hamper endophyte isolation [ 22 ].

Therefore, the selection of isolation media and surface sterilization are important to maximize the successful isolation of endophytic actinomycetes, thus, enhancing the possibility to discover new endophytes [ 23 ]. Three medicinal plants have been chosen for the present study i.

endophytic actinomycetes

Thai or sweet basil is a common name for the culinary herb O. It is considered to be antibacterial, antifungal, antispasmodic, carminative, diaphoretic, digestive and expectorant [ 24 ].

This is the first report of the isolation of endophytic actinomycetes and till now, there are no reports on endophytic actinomycetes from O. The present study was carried out to endophytic actinomycetes the effectiveness of surface sterilization methods and standardization of endophytic actinomycetes suitable growth and purified media.

This endophytic actinomycetes is endophytic actinomycetes part of larger study which relates the presence of endophytic actinomycetes in medicinal plants and gives the distinctive features to medicinal value of the plants.

Materials and Methods Selection and collection of medicinal plants Important regional medicinal plants O. Plants were sampled over growing seasons May to February.


All the three experimental medicinal plants were sampled from botanical garden of Karnatak University, Dharwad. At each sampling events, a total of 10 plants were dug out carefully endophytic actinomycetes ensure that maximal amount of roots material were collected.

Two batches of five plants endophytic actinomycetes collected at interval of 10 min.