THROUGHPUT DIELECTROPHORETIC. APPLICATIONS a thesis submitted to Keywords: Microfludics, Electrokinetic, Dielectrophoresis, Particle Separation. Dielectrophoresis of colloids and polyelectrolytes by. David John Guy Bakewell. A thesis submitted to the Faculty of Engineering. University of Glasgow. This thesis presents models and simulations of dielectrophoretic separation Dielectrophoresis,micro particle, molecular dynamics, single-walled carbon nan-.


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Suspensions consisting of single particle types and a mixture were then evaluated, and dielectrophoresis thesis was found that the particles collected in the regions specified by the theoretical calculations.


These results showed dielectrophoresis thesis the device is capable of collecting particles based on the dielectric properties of the magnetite particles dielectrophoresis thesis polystyrene spheres.

We observe that when electroporation is performed in media with lower ionic concentration than cells cytoplasm, their internal conductivity decreases after electroporation depending on the intensity of applied pulses.

We hypothesize the reason is the presence of large and relatively immobile negative ions inside the cell which attract mobile positive ions mainly sodium and potassium to dielectrophoresis thesis cell electrical neutrality.

We monitor the temporal response of cells after electroporation to measure the time constant of changes due to ion transport and observe this ranges from seconds to tens of seconds depending on dielectrophoresis thesis applied pulse intensity. In this thesis we dielectrophoresis thesis a dielectrophoresis DEP based technique to study changes in the cytoplasm conductivity of single Chinese hamster ovary CHO cells immediately after electroporation.

Using a microfluidic chip, we study changes in the DEP response of single CHO cells a few seconds after electroporation.

  • Dielectrophoresis study of electroporation effects on dielectric properties of biological cells
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  • Dielectrophoresis study of electroporation effects on dielectric properties of biological cells

First, in order to quantify our DEP measurement results and relate them to the cells internal conductivity, we introduce dielectrophoresis thesis dielectric model for CHO cells. Lithography based manufacturing techniques were developed to produce suitable electrodes for dielectrophoresis on a scale small enough to manipulate sub-micrometre particles.

Detailed electric field patterns were numerically calculated for these electrodes, so that the dielectrophoretic force could dielectrophoresis thesis simulated, predicted and compared with experimental measurements of dielectrophoresis thesis movement.